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Introduces the importance of preparing a true single cell suspension before cell separation. Explains that this improves labeling efficiency and reduces cell loss.
one of the most important factors in successful cell isolations happens before you even start your separation preparing a true single cell suspension of your starting sample will optimize your cell separations by avoiding additional cell loss and enabling maximumlabeling of the target cells this video outlines a method to harvest cells from a primary tissue sample and prepare a single cell suspension prior to performing cell separation when working with primary tissue samples cells must be dissociated from
Describes how to mince primary tissue, dissociate it in medium, and pass it through a mesh strainer. Covers washing the strainer and collecting the cells by centrifugation.
the tissue in order to prepare a single cell suspension in a sterile dish containing dissociation medium mince the harvested tissue place a mesh strainer on a sterile 50 mil conical tube a 70 or 100 Micron cell strainer workswell transfer the dissociated tissue and the dissociated media to the mesh strainer gently pass the dissociated tissue through the strainer wash the tissue in cells in the strainer by gently adding a few milliliters of PBS repeat this stepand then discard the leftover tissue and strainer top up the tube containing the cell suspension with PBS and collect the cells by centrifugation
Explains removing the supernatant, resuspending the pellet, and optionally washing the cells. Concludes with the suspension ready for counting and EasySep processing.
carefully remove and discard the supernant without disturbing the pallet gently tap the tube to resuspend the pellet You may wish to do an optional wash at this stage you should now have a single cell suspension that is readyfor cell counting and subsequent processing using easysep to isolate your desired cells for more information on preparing single cell suspensions from primary tissue samples and other procedures please visit our website at stem cell.com